Review



human mda mb 231 bc cell line  (ATCC)


Bioz Verified Symbol ATCC is a verified supplier
Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 99
      Buy from Supplier

    Structured Review

    ATCC human mda mb 231 bc cell line
    Human Mda Mb 231 Bc Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 24590 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+bc+cell+line+mda+mb+231/MDA-MB-231/pm41957669-100-1-6
    Average 99 stars, based on 24590 article reviews
    human mda mb 231 bc cell line - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Multiple Displacement Amplification:

    Article Title: The DAG/PKC/CREB1/TGF-β1 axis drives shear-wave elastography stiffness and malignant progression in triple-negative breast cancer via lipid metabolic reprogramming
    Article Snippet: .. Human BC cell line MDA-MB-231 and murine BC cell lines E0771, 4T1, and AT3 were purchased from the American Type Culture Collection (ATCC). .. 4T1-luc and NIH-3T3 cells were obtained from QuiCell (Shanghai, China).

    Article Title: Breast Cancer Cell Line-Specific Responses to Insulin: Effects on Proliferation and Migration
    Article Snippet: .. The human BC cell line MDA-MB-231 (negative for oestrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2)) was obtained from LGC Standards (Middlesex, UK), while the cell line MCF-7 (positive for ER and PR but negative for HER2) [ ] was purchased from the American Type Culture Collection (Rockville, MD, USA). .. Both the cell lines were cultured in Dulbecco′s Modified Eagle′s Medium (DMEM)-Low Glucose (5.5 mM glucose) (Euroclone, Pero, MI, Italy) supplemented with 100 U/mL Penicillin-Streptomycin, both purchased from Sigma-Aldrich (St. Louis, MO, USA), and 10% Foetal Bovine Serum (FBS) obtained from Microgen (Microgem, Naples, NA, Italy) and incubated at 5% CO 2 and 90% relative humidity at 37 °C.

    Article Title: Bone Marrow Myeloid–Lymphatic Progenitors Expand Tumor Lymphatic Vasculature Through Cell Fusion
    Article Snippet: .. Human BC cell line MDA-MB-231 and mouse EMT6 were obtained from ATCC (Manassas, VA, USA) and engineered for stable expression of luciferase (Luc) as described previously [ ]. ..

    Article Title: Tumor‐derived exosomal miR ‐148b‐3p mediates M2 macrophage polarization via TSC2 / mTORC1 to promote breast cancer migration and invasion
    Article Snippet: .. Human BC cell line MDA‐MB‐231, human breast epithelial cell line MCF‐10A and human leukemic cell line THP‐1 were all acquired from American Type Culture Collection (ATCC). ..

    Article Title: The DAG/PKC/CREB1/TGF-β1 axis drives shear-wave elastography stiffness and malignant progression in triple-negative breast cancer via lipid metabolic reprogramming.
    Article Snippet: .. Cell lines and Culture conditions: Human BC cell line MDA-MB-231 and murine B C cell lines E0771, 4T1, and AT3 were purchased from the American Type Cultur e Collection (ATCC). .. 4T1-luc and NIH-3T3 cells were obtained from QuiCell (Shan ghai, China).

    Article Title: Bone Marrow Myeloid-Lymphatic Progenitors Expand Tumor Lymphatic Vasculature Through Cell Fusion.
    Article Snippet: .. Human BC cell line MDA-MB-231 and mouse EMT6 were obtained from ATCC (Manassas, VA, USA) and engineered for stable expression of luciferase (Luc) as described previously [54]. ..

    Article Title: Crosstalk between miR-203 and PKCθ regulates breast cancer stem cell markers.
    Article Snippet: Correspondence Sohair Salem,MolecularGenetics and EnzymologyDepartment,National ResearchCentre, 33El-BuhouthSt.Dokki, Giza 12622, Egypt.. Email: ssalem_nrc@hotmail.com Abstract Introduction: Protein kinase C theta (PKCθ) is expressed in ER-negative breast cancer and promotes cancer stem cells (CSCs) phenotype.. PKCθ gene (PRKCQ) is predicted to be a target for tumor suppressormiR-203.

    Article Title: Antitumoral Activity and Metabolic Signatures of Dichloroacetate, 6-Aminonicotinamide and Etomoxir in Breast-Tumor-Educated Macrophages.
    Article Snippet: Pharmacological targeting of metabolic pathways represents an appealing strategy to selectively kill cancer cells while promoting antitumor functions of stromal cells.. In this study, we assessed the effectiveness of 13 metabolic drugs (MDs) in steering in vitro generated breast tumor-educated macrophages (TEMs) toward an antitumoral phenotype.. For that, the production of vascular endothelial growth factor (VEGF) and tumor necrosis factor α (TNF-α), two important regulators of tumor progression, was evaluated.

    Expressing:

    Article Title: Bone Marrow Myeloid–Lymphatic Progenitors Expand Tumor Lymphatic Vasculature Through Cell Fusion
    Article Snippet: .. Human BC cell line MDA-MB-231 and mouse EMT6 were obtained from ATCC (Manassas, VA, USA) and engineered for stable expression of luciferase (Luc) as described previously [ ]. ..

    Article Title: Bone Marrow Myeloid-Lymphatic Progenitors Expand Tumor Lymphatic Vasculature Through Cell Fusion.
    Article Snippet: .. Human BC cell line MDA-MB-231 and mouse EMT6 were obtained from ATCC (Manassas, VA, USA) and engineered for stable expression of luciferase (Luc) as described previously [54]. ..

    Luciferase:

    Article Title: Bone Marrow Myeloid–Lymphatic Progenitors Expand Tumor Lymphatic Vasculature Through Cell Fusion
    Article Snippet: .. Human BC cell line MDA-MB-231 and mouse EMT6 were obtained from ATCC (Manassas, VA, USA) and engineered for stable expression of luciferase (Luc) as described previously [ ]. ..

    Article Title: Bone Marrow Myeloid-Lymphatic Progenitors Expand Tumor Lymphatic Vasculature Through Cell Fusion.
    Article Snippet: .. Human BC cell line MDA-MB-231 and mouse EMT6 were obtained from ATCC (Manassas, VA, USA) and engineered for stable expression of luciferase (Luc) as described previously [54]. ..



    Similar Products

    human mda mb 231 bc cell line  (ATCC)
    99
    ATCC human mda mb 231 bc cell line
    Human Mda Mb 231 Bc Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+bc+cell+line+mda+mb+231/MDA-MB-231/pm41957669-100-1-6
    Average 99 stars, based on 1 article reviews
    human mda mb 231 bc cell line - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier
    mda mb 231 human bc cell line  (Pasteur Institute)
    86
    Pasteur Institute mda mb 231 human bc cell line
    Mda Mb 231 Human Bc Cell Line, supplied by Pasteur Institute, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+bc+cell+line+mda+mb+231/231+mb+mda/pm42087141-75-3-19
    Average 86 stars, based on 1 article reviews
    mda mb 231 human bc cell line - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier
    human bc cell line mda mb 231  (ATCC)
    99
    ATCC human bc cell line mda mb 231
    A Cell viability measured by CCK-8 <t>assay</t> <t>in</t> <t>MDA-MB-231</t> and E0771 cells treated with increasing concentrations of Sotrastaurin. Western blot analysis of PKC, phosphorylated PKC (p-PKC), and epithelial–mesenchymal transition (EMT)-related markers in MDA-MB-231 ( B ) and E0771 ( C ) cells after treatment with Diolein and Sotrastaurin. D BODIPY staining and quantitative analysis of lipid droplets in MDA-MB-231 cells treated with Diolein. Scale bars, 50 μm. Quantitative results of colony formation assays in MDA-MB-231 ( E ) and E0771 ( F ) cells treated with Diolein and Sotrastaurin. Wound healing assay and quantitative analysis of migration in MDA-MB-231 ( G ) and E0771 ( H ) cells treated with Diolein and Sotrastaurin. Scale bars, 100 μm. Transwell invasion and migration assays with quantitative results in MDA-MB-231 ( I ) and E0771 ( J ) cells after treatment with Diolein and Sotrastaurin. Scale bars, 100 μm. Data are presented as mean ± SEM.
    Human Bc Cell Line Mda Mb 231, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+bc+cell+line+mda+mb+231/MDA-MB-231/pmc13039978-346-0-18
    Average 99 stars, based on 1 article reviews
    human bc cell line mda mb 231 - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier
    human bc cell lines  (ATCC)
    99
    ATCC human bc cell lines
    A Cell viability measured by CCK-8 <t>assay</t> <t>in</t> <t>MDA-MB-231</t> and E0771 cells treated with increasing concentrations of Sotrastaurin. Western blot analysis of PKC, phosphorylated PKC (p-PKC), and epithelial–mesenchymal transition (EMT)-related markers in MDA-MB-231 ( B ) and E0771 ( C ) cells after treatment with Diolein and Sotrastaurin. D BODIPY staining and quantitative analysis of lipid droplets in MDA-MB-231 cells treated with Diolein. Scale bars, 50 μm. Quantitative results of colony formation assays in MDA-MB-231 ( E ) and E0771 ( F ) cells treated with Diolein and Sotrastaurin. Wound healing assay and quantitative analysis of migration in MDA-MB-231 ( G ) and E0771 ( H ) cells treated with Diolein and Sotrastaurin. Scale bars, 100 μm. Transwell invasion and migration assays with quantitative results in MDA-MB-231 ( I ) and E0771 ( J ) cells after treatment with Diolein and Sotrastaurin. Scale bars, 100 μm. Data are presented as mean ± SEM.
    Human Bc Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+bc+cell+line+mda+mb+231/MDA-MB-231/pm41582764-97-0-11
    Average 99 stars, based on 1 article reviews
    human bc cell lines - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier
    human bc cell lines mda mb 231  (ATCC)
    99
    ATCC human bc cell lines mda mb 231
    A Cell viability measured by CCK-8 <t>assay</t> <t>in</t> <t>MDA-MB-231</t> and E0771 cells treated with increasing concentrations of Sotrastaurin. Western blot analysis of PKC, phosphorylated PKC (p-PKC), and epithelial–mesenchymal transition (EMT)-related markers in MDA-MB-231 ( B ) and E0771 ( C ) cells after treatment with Diolein and Sotrastaurin. D BODIPY staining and quantitative analysis of lipid droplets in MDA-MB-231 cells treated with Diolein. Scale bars, 50 μm. Quantitative results of colony formation assays in MDA-MB-231 ( E ) and E0771 ( F ) cells treated with Diolein and Sotrastaurin. Wound healing assay and quantitative analysis of migration in MDA-MB-231 ( G ) and E0771 ( H ) cells treated with Diolein and Sotrastaurin. Scale bars, 100 μm. Transwell invasion and migration assays with quantitative results in MDA-MB-231 ( I ) and E0771 ( J ) cells after treatment with Diolein and Sotrastaurin. Scale bars, 100 μm. Data are presented as mean ± SEM.
    Human Bc Cell Lines Mda Mb 231, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+bc+cell+line+mda+mb+231/MDA-MB-231/pmc12734722-86-1-12
    Average 99 stars, based on 1 article reviews
    human bc cell lines mda mb 231 - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier
    human bc cell lines mda mb 231  (Procell Inc)
    86
    Procell Inc human bc cell lines mda mb 231
    A Cell viability measured by CCK-8 <t>assay</t> <t>in</t> <t>MDA-MB-231</t> and E0771 cells treated with increasing concentrations of Sotrastaurin. Western blot analysis of PKC, phosphorylated PKC (p-PKC), and epithelial–mesenchymal transition (EMT)-related markers in MDA-MB-231 ( B ) and E0771 ( C ) cells after treatment with Diolein and Sotrastaurin. D BODIPY staining and quantitative analysis of lipid droplets in MDA-MB-231 cells treated with Diolein. Scale bars, 50 μm. Quantitative results of colony formation assays in MDA-MB-231 ( E ) and E0771 ( F ) cells treated with Diolein and Sotrastaurin. Wound healing assay and quantitative analysis of migration in MDA-MB-231 ( G ) and E0771 ( H ) cells treated with Diolein and Sotrastaurin. Scale bars, 100 μm. Transwell invasion and migration assays with quantitative results in MDA-MB-231 ( I ) and E0771 ( J ) cells after treatment with Diolein and Sotrastaurin. Scale bars, 100 μm. Data are presented as mean ± SEM.
    Human Bc Cell Lines Mda Mb 231, supplied by Procell Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+bc+cell+line+mda+mb+231/231+mb+mda/pmc12308457-76-1-24
    Average 86 stars, based on 1 article reviews
    human bc cell lines mda mb 231 - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier
    culture human bc cell lines mda mb 231  (ATCC)
    99
    ATCC culture human bc cell lines mda mb 231
    A Cell viability measured by CCK-8 <t>assay</t> <t>in</t> <t>MDA-MB-231</t> and E0771 cells treated with increasing concentrations of Sotrastaurin. Western blot analysis of PKC, phosphorylated PKC (p-PKC), and epithelial–mesenchymal transition (EMT)-related markers in MDA-MB-231 ( B ) and E0771 ( C ) cells after treatment with Diolein and Sotrastaurin. D BODIPY staining and quantitative analysis of lipid droplets in MDA-MB-231 cells treated with Diolein. Scale bars, 50 μm. Quantitative results of colony formation assays in MDA-MB-231 ( E ) and E0771 ( F ) cells treated with Diolein and Sotrastaurin. Wound healing assay and quantitative analysis of migration in MDA-MB-231 ( G ) and E0771 ( H ) cells treated with Diolein and Sotrastaurin. Scale bars, 100 μm. Transwell invasion and migration assays with quantitative results in MDA-MB-231 ( I ) and E0771 ( J ) cells after treatment with Diolein and Sotrastaurin. Scale bars, 100 μm. Data are presented as mean ± SEM.
    Culture Human Bc Cell Lines Mda Mb 231, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+bc+cell+line+mda+mb+231/MDA-MB-231/pm40530439-50-3-15
    Average 99 stars, based on 1 article reviews
    culture human bc cell lines mda mb 231 - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    A Cell viability measured by CCK-8 assay in MDA-MB-231 and E0771 cells treated with increasing concentrations of Sotrastaurin. Western blot analysis of PKC, phosphorylated PKC (p-PKC), and epithelial–mesenchymal transition (EMT)-related markers in MDA-MB-231 ( B ) and E0771 ( C ) cells after treatment with Diolein and Sotrastaurin. D BODIPY staining and quantitative analysis of lipid droplets in MDA-MB-231 cells treated with Diolein. Scale bars, 50 μm. Quantitative results of colony formation assays in MDA-MB-231 ( E ) and E0771 ( F ) cells treated with Diolein and Sotrastaurin. Wound healing assay and quantitative analysis of migration in MDA-MB-231 ( G ) and E0771 ( H ) cells treated with Diolein and Sotrastaurin. Scale bars, 100 μm. Transwell invasion and migration assays with quantitative results in MDA-MB-231 ( I ) and E0771 ( J ) cells after treatment with Diolein and Sotrastaurin. Scale bars, 100 μm. Data are presented as mean ± SEM.

    Journal: Cell Death & Disease

    Article Title: The DAG/PKC/CREB1/TGF-β1 axis drives shear-wave elastography stiffness and malignant progression in triple-negative breast cancer via lipid metabolic reprogramming

    doi: 10.1038/s41419-026-08625-0

    Figure Lengend Snippet: A Cell viability measured by CCK-8 assay in MDA-MB-231 and E0771 cells treated with increasing concentrations of Sotrastaurin. Western blot analysis of PKC, phosphorylated PKC (p-PKC), and epithelial–mesenchymal transition (EMT)-related markers in MDA-MB-231 ( B ) and E0771 ( C ) cells after treatment with Diolein and Sotrastaurin. D BODIPY staining and quantitative analysis of lipid droplets in MDA-MB-231 cells treated with Diolein. Scale bars, 50 μm. Quantitative results of colony formation assays in MDA-MB-231 ( E ) and E0771 ( F ) cells treated with Diolein and Sotrastaurin. Wound healing assay and quantitative analysis of migration in MDA-MB-231 ( G ) and E0771 ( H ) cells treated with Diolein and Sotrastaurin. Scale bars, 100 μm. Transwell invasion and migration assays with quantitative results in MDA-MB-231 ( I ) and E0771 ( J ) cells after treatment with Diolein and Sotrastaurin. Scale bars, 100 μm. Data are presented as mean ± SEM.

    Article Snippet: Human BC cell line MDA-MB-231 and murine BC cell lines E0771, 4T1, and AT3 were purchased from the American Type Culture Collection (ATCC).

    Techniques: CCK-8 Assay, Western Blot, Staining, Wound Healing Assay, Migration

    A CREB1 mRNA expression was downregulated in Sotrastaurin-treated MDA-MB-231 cells compared to controls, as determined by RNA-seq. B Analysis of the GEO dataset GSE137467 confirmed that PKC inhibitor treatment also reduced CREB1 mRNA levels in this cell line. Western blot analysis of CREB1 and phosphorylated CREB1 (p-CREB1) in MDA-MB-231 ( C ) and E0771 ( D ) cells treated with Diolein and Sotrastaurin. E TGF-β1 mRNA expression was downregulated in Sotrastaurin-treated MDA-MB-231 cells compared to controls, as determined by RNA-seq. F Western blot analysis of CREB1, p-CREB1, TGF-β1, MMP9 and N-cadherin in MDA-MB-231 treated with siCREB1 and Diolein. G Western blot analysis of CREB1, p-CREB1, and TGF-β1 in MDA-MB-231 ( G ) and E0771 ( H ) cells treated with PMA (PKC activator) and 666-15 (CREB1 inhibitor). I Western blot analysis of EMT-related markers in MDA-MB-231 cells treated with 666-15 and SRI-011381 (TGF-β1 activator). Quantitative results of colony formation assays ( J ), wound healing assay ( K ) and transwell invasion and migration assays ( L ) in MDA-MB-231 cells treated with siCREB1 and Diolein. Scale bars 100 μm. Quantitative results of colony formation assays in MDA-MB-231 ( M ) and E0771 ( N ) cells treated with 666-15 and SRI-011381. Wound healing assay and quantitative analysis of migration in MDA-MB-231 ( O ) and E0771 ( P ) cells treated with 666-15 and SRI-011381. Scale bars, 100 μm. Transwell invasion and migration assays with quantitative results in MDA-MB-231 ( Q ) and E0771 ( R ) cells after treatment with 666-15 and SRI-011381. Scale bars, 100μm. Data are presented as mean ± SEM.

    Journal: Cell Death & Disease

    Article Title: The DAG/PKC/CREB1/TGF-β1 axis drives shear-wave elastography stiffness and malignant progression in triple-negative breast cancer via lipid metabolic reprogramming

    doi: 10.1038/s41419-026-08625-0

    Figure Lengend Snippet: A CREB1 mRNA expression was downregulated in Sotrastaurin-treated MDA-MB-231 cells compared to controls, as determined by RNA-seq. B Analysis of the GEO dataset GSE137467 confirmed that PKC inhibitor treatment also reduced CREB1 mRNA levels in this cell line. Western blot analysis of CREB1 and phosphorylated CREB1 (p-CREB1) in MDA-MB-231 ( C ) and E0771 ( D ) cells treated with Diolein and Sotrastaurin. E TGF-β1 mRNA expression was downregulated in Sotrastaurin-treated MDA-MB-231 cells compared to controls, as determined by RNA-seq. F Western blot analysis of CREB1, p-CREB1, TGF-β1, MMP9 and N-cadherin in MDA-MB-231 treated with siCREB1 and Diolein. G Western blot analysis of CREB1, p-CREB1, and TGF-β1 in MDA-MB-231 ( G ) and E0771 ( H ) cells treated with PMA (PKC activator) and 666-15 (CREB1 inhibitor). I Western blot analysis of EMT-related markers in MDA-MB-231 cells treated with 666-15 and SRI-011381 (TGF-β1 activator). Quantitative results of colony formation assays ( J ), wound healing assay ( K ) and transwell invasion and migration assays ( L ) in MDA-MB-231 cells treated with siCREB1 and Diolein. Scale bars 100 μm. Quantitative results of colony formation assays in MDA-MB-231 ( M ) and E0771 ( N ) cells treated with 666-15 and SRI-011381. Wound healing assay and quantitative analysis of migration in MDA-MB-231 ( O ) and E0771 ( P ) cells treated with 666-15 and SRI-011381. Scale bars, 100 μm. Transwell invasion and migration assays with quantitative results in MDA-MB-231 ( Q ) and E0771 ( R ) cells after treatment with 666-15 and SRI-011381. Scale bars, 100μm. Data are presented as mean ± SEM.

    Article Snippet: Human BC cell line MDA-MB-231 and murine BC cell lines E0771, 4T1, and AT3 were purchased from the American Type Culture Collection (ATCC).

    Techniques: Expressing, RNA Sequencing, Western Blot, Wound Healing Assay, Migration

    A Western blot analysis of COL1A1 and α-SMA expression in NIH-3T3 cells treated with control, conditioned medium from Diolein-treated E0771 cells (CM(D-7)), or CM(D-7) supplemented with P144 (TGF-β1 inhibitor). B Immunofluorescence staining and quantitative analysis of COL1A1 and α-SMA in NIH-3T3 cells under the same treatment conditions as in ( A ). Scale bar, 50μm. C TGF-β1 secretion levels measured by ELISA in NIH-3T3 cells cultured with different conditioned media. D Phalloidin staining of E0771 cells treated with control, conditioned medium from NIH-3T3 exposed to CM(D-7) (CM(D-7-3)), CM(D-7-3) + P144, or recombinant TGF-β1. Scale bar, 20μm. E BODIPY staining and quantitative analysis of lipid droplets in E0771 cells treated with control, Diolein, conditioned medium from NIH-3T3 (CM(3)), or CM(D-7-3). Scale bar, 50 μm. F Measurement of triglyceride and cholesterol levels in E0771 cells treated with control or CM(D-7-3). G Western blot analysis of lipid metabolism markers (FASN, SREBP, PPARγ) in E0771 cells treated with control, CM(3), conditioned medium from E0771-exposed NIH-3T3 (CM(7-3)), or CM(D-7-3). H Transwell invasion and migration assays with quantitative results in MDA-MB-231 and E0771 cells treated with control or CM(D-7-3). Scale bar, 100 μm. Data are presented as mean ± SEM.

    Journal: Cell Death & Disease

    Article Title: The DAG/PKC/CREB1/TGF-β1 axis drives shear-wave elastography stiffness and malignant progression in triple-negative breast cancer via lipid metabolic reprogramming

    doi: 10.1038/s41419-026-08625-0

    Figure Lengend Snippet: A Western blot analysis of COL1A1 and α-SMA expression in NIH-3T3 cells treated with control, conditioned medium from Diolein-treated E0771 cells (CM(D-7)), or CM(D-7) supplemented with P144 (TGF-β1 inhibitor). B Immunofluorescence staining and quantitative analysis of COL1A1 and α-SMA in NIH-3T3 cells under the same treatment conditions as in ( A ). Scale bar, 50μm. C TGF-β1 secretion levels measured by ELISA in NIH-3T3 cells cultured with different conditioned media. D Phalloidin staining of E0771 cells treated with control, conditioned medium from NIH-3T3 exposed to CM(D-7) (CM(D-7-3)), CM(D-7-3) + P144, or recombinant TGF-β1. Scale bar, 20μm. E BODIPY staining and quantitative analysis of lipid droplets in E0771 cells treated with control, Diolein, conditioned medium from NIH-3T3 (CM(3)), or CM(D-7-3). Scale bar, 50 μm. F Measurement of triglyceride and cholesterol levels in E0771 cells treated with control or CM(D-7-3). G Western blot analysis of lipid metabolism markers (FASN, SREBP, PPARγ) in E0771 cells treated with control, CM(3), conditioned medium from E0771-exposed NIH-3T3 (CM(7-3)), or CM(D-7-3). H Transwell invasion and migration assays with quantitative results in MDA-MB-231 and E0771 cells treated with control or CM(D-7-3). Scale bar, 100 μm. Data are presented as mean ± SEM.

    Article Snippet: Human BC cell line MDA-MB-231 and murine BC cell lines E0771, 4T1, and AT3 were purchased from the American Type Culture Collection (ATCC).

    Techniques: Western Blot, Expressing, Control, Immunofluorescence, Staining, Enzyme-linked Immunosorbent Assay, Cell Culture, Recombinant, Migration

    A SWE and B-mode ultrasound images of tumors from the E0771 model. ( n = 10 per group). Quantitative analysis of SWE-based tumor stiffness ( B ), LOX levels (measured by ELISA) ( C ), α-SMA IHC ( D ) and Sirius Red staining ( E ) in tumor tissues from the E0771 model. Quantitative analysis of SWE-based tumor stiffness ( F ), LOX levels (measured by ELISA) ( G ), α-SMA IHC ( H ) and Sirius Red staining ( I ) in tumor tissues from the AT3 model. Quantitative analysis of SWE-based tumor stiffness ( J ), LOX levels (measured by ELISA) ( K ), α-SMA IHC ( L ) and Sirius Red staining ( M ) in tumor tissues from the MDA-MB-231 model. Data are presented as mean ± SEM.

    Journal: Cell Death & Disease

    Article Title: The DAG/PKC/CREB1/TGF-β1 axis drives shear-wave elastography stiffness and malignant progression in triple-negative breast cancer via lipid metabolic reprogramming

    doi: 10.1038/s41419-026-08625-0

    Figure Lengend Snippet: A SWE and B-mode ultrasound images of tumors from the E0771 model. ( n = 10 per group). Quantitative analysis of SWE-based tumor stiffness ( B ), LOX levels (measured by ELISA) ( C ), α-SMA IHC ( D ) and Sirius Red staining ( E ) in tumor tissues from the E0771 model. Quantitative analysis of SWE-based tumor stiffness ( F ), LOX levels (measured by ELISA) ( G ), α-SMA IHC ( H ) and Sirius Red staining ( I ) in tumor tissues from the AT3 model. Quantitative analysis of SWE-based tumor stiffness ( J ), LOX levels (measured by ELISA) ( K ), α-SMA IHC ( L ) and Sirius Red staining ( M ) in tumor tissues from the MDA-MB-231 model. Data are presented as mean ± SEM.

    Article Snippet: Human BC cell line MDA-MB-231 and murine BC cell lines E0771, 4T1, and AT3 were purchased from the American Type Culture Collection (ATCC).

    Techniques: Enzyme-linked Immunosorbent Assay, Staining